Journal: Scientific Reports

Article Title: CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis -infected Cattle

doi: 10.1038/srep29990

Figure Lengend Snippet: Summary of flow cytometry antibody labelling panels a .

Article Snippet: CD4+ T cell population , 1 ° : anti-boCD4-AF647 , 1 ° : boil-22 Fab antibody (clone 58, Bio-Rad AbD Serotec), polyclonal rabbit anti-boIL-17A-biotin (Kingfisher Biotech)2 ° : anti-huIgG F(ab’) 2 -FITC, Streptavidin-PE (BD Pharmingen).

Techniques: Flow Cytometry, Clone Assay, Recombinant

Journal: BMC Veterinary Research

Article Title: Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

doi: 10.1186/s12917-018-1765-9

Figure Lengend Snippet: Cellular immune responses following stimulation with LukM. IFNg ( a ) and IL17a ( c ) production following stimulation of whole blood with LukM for 48 h and 72 h, respectively. Proliferation measured as the percentage of CD4 ( b ) and CD8 ( d ) T-cells with diluted CFSE signal following 96 h stimulation with LukM. S/P = Sample to positive ratio. + = P < 0,05 before correction for multiple comparisons. * = P < 0,05 after correction for multiple comparisons

Article Snippet: Subsequently cells were stained with a biotinylated anti-bovine IFN-γ monoclonal antibody (6C3; BioSource, San Diego, California, USA) or biotinylated anti-bovine IL17a polycolonal antibodies (Kingfisher Biotech, Inc.) for 30 min at 4 °C in the dark.

Techniques:

Journal: BMC Veterinary Research

Article Title: Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

doi: 10.1186/s12917-018-1765-9

Figure Lengend Snippet: Cellular immune responses following stimulation with EfB. IFNg ( a ) and IL17a ( c ) production following stimulation of whole blood with EfB for 48 h and 72 h, respectively. Proliferation measured as the percentage of CD4 ( b ) and CD8 ( d ) T-cells with diluted CFSE signal following 96 h stimulation with EfB. S/P = Sample to positive ratio. + = P < 0,05 before correction for multiple comparisons. * = P < 0,05 after correction for multiple comparisons

Article Snippet: Subsequently cells were stained with a biotinylated anti-bovine IFN-γ monoclonal antibody (6C3; BioSource, San Diego, California, USA) or biotinylated anti-bovine IL17a polycolonal antibodies (Kingfisher Biotech, Inc.) for 30 min at 4 °C in the dark.

Techniques:

Journal: Tropical Animal Health and Production

Article Title: Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia

doi: 10.1007/s11250-016-0994-9

Figure Lengend Snippet: Semi-quantitative assessment of inflammatory cytokines in the lung of infected and uninfected animals

Article Snippet: Bovine IL-1β, IL-17A, and TNF-α were detected by using biotinylated rabbit anti-bovine IL-1β polyclonal antibody (article no. ab23778, Abcam, UK), biotinylated rabbit anti-bovine IL-17A polyclonal antibody (article no. PBB0277B-050, Kingfisher Biotech, Inc., USA), and biotinylated rabbit anti-bovine TNF-α polyclonal antibodies (article no. AHP852B, AbD Serotec, Germany), respectively.

Techniques: Infection, Negative Control

Journal: Tropical Animal Health and Production

Article Title: Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia

doi: 10.1007/s11250-016-0994-9

Figure Lengend Snippet: Representative qualitative results of the detection of the cytokines IL-1β, IL-17A, and TNF-α. The following polyclonal antibodies were used. a Anti- IL-1β: Lung: control animal; b Anti- IL-1β: Lung: acute CBPP lesion; c Anti- IL-17A: Lung: control animal; d Anti- IL-17A: Lung: acute CBPP lesion; e Anti- TNF-α: Lung: control animal; f Anti- TNF-α: Lung: acute CBPP lesion; g Anti- Mycoplasma : Lung: control animal; h Anti- Mycoplasma : Lung: acute CBPP lesion. Black bars in the lower right corner of images in a – f is the size standard representing 100 μm, black bars in images of g , h represent 20 μm. Arrows indicate positive immunoreactive signals ( reddish-brown )

Article Snippet: Bovine IL-1β, IL-17A, and TNF-α were detected by using biotinylated rabbit anti-bovine IL-1β polyclonal antibody (article no. ab23778, Abcam, UK), biotinylated rabbit anti-bovine IL-17A polyclonal antibody (article no. PBB0277B-050, Kingfisher Biotech, Inc., USA), and biotinylated rabbit anti-bovine TNF-α polyclonal antibodies (article no. AHP852B, AbD Serotec, Germany), respectively.

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis

doi: 10.3389/fimmu.2015.00628

Figure Lengend Snippet: Lymph node IFN-γ and IL-17A response to Hirep1 re-stimulation at day 15 pi .

Article Snippet: IL-17A was detected with a rabbit anti-pig IL-17A polyclonal-coating antibody (KP0498S-100, Kingfischer Biotech, Saint Paul) (1 μg/ml) biotinylated rabbit anti-pig IL-17A polyclonal detection antibody (KPB0499S-050, Kingfisher Biotech, Saint Paul) in 0.1 μg/ml and HRP-conjugated streptavidin (SNN1004, Life TechnologiesTM, Denmark) in 0.1 μg/ml.

Techniques: